![]() ![]() It is concluded that the estimated molecular weight of SMOBIO’s pre-stained marker shows a curve matching well with that of unstained native proteins (MARK12), representing a good estimation of the MW of each pre-stained protein in the SDS-PAGE analysis. Although it is impossible to define "precision" for molecular weight of proteins in SDS-PAGE, we did compare the migration pattern of pre-stained markers with unstained protein marker (Invitrogen MARK12) for calibration. Therefore, in the product description, we suggest our users to calibrate the MW against their interested proteins. We did compare the migration patterns of SMOBIO’s Protein Markers with other brands, and we concluded that it was difficult to define “precision” due to the reasons mentioned above. For example, a protein which is highly hydrophilic might show a particular higher position in the SDS-PAGE analysis when compared to a hydrophobic one. It is known that the analysis of protein size by an SDS-PAGE is only for “estimation” because of the intrinsic variation of amino acid composition in all proteins including stained and non-stained ones. Usually, pre-stained marker is written on “estimated molecular weight” for caution. MOPS, and MES buffer) and after Western blotting to nitrocellulose membrane. Under suggested conditions, PM5200 ExcelBand™ 3-color Pre-Stained Protein Ladder Broad Range resolves 15 major bands in SDS-PAGE ( Tris-Glycine, Three reference bands - 75 kDa (red), 40 kDa (green), and 20 kDa (blue)Īpproximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25☌), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol). Ready-to-use - Premixed with a loading buffer for direct loading, no need to boil. Protein ladders, Protein size marker, SDS-PAGE, Western blotting. Use the ladder for easy visualization of gel migration and protein size, and to orient your gel or. western blot Our prestained and immunodetectable WesternFroxx all-in-one Protein Ladder. The PM5200 3-color Pre-Stained Protein Ladder Broad Range is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. Protein Molecular Weight Marker EXtended is ideal for precise sizing of proteins by SDS-PAGE and for Western blotting. Includes 11 protein bands from 8 to 260 kDa (see figure). Proteins are covalently coupled with different chromophores for easy identification of bands, with three reference proteins carrying enhanced intensity corresponding to a blue band at 20 kDa, green at 40 kDa, and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). Not intended for human or animal diagnostic or therapeutic uses.The PM5200 3-color Pre-Stained Protein Ladder Broad Range is a ready-to-use three-color protein standard with 15 pre-stained proteins covering a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine Buffer (3.5 to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards supplemental data should be considered for more accurate adjustment in different electrophoresis conditions. Under suggested conditions, Unveil Unstained Protein Ladder resolves 12 major bands in 4-12%Bis- Tris Gel(MES buffer) and after Western blotting to the nitrocellulose membrane. 2.5 μl per well for general Western transferring.Ģ0 mM Tris-phosphate(pH 7.5 at 25☌), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol. The ladder is supplied in the gel loading buffer and is ready to use.ģ μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. The Unveil Unstained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The proteins resolve into clearly identifiable bands when separated on the SDS-PAGE gel (MES buffer), with the intensified 25kDa and 85kDa protein bands serving as the two reference bands. nine blue-, orange- and green-stained proteins (10 to 250 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting. The Unveil Unstained Protein Ladder is a mixture of 12 unstained recombinant proteins covering a wide range of molecular weights from 10 to 200 kDa.
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